Cloning, Expression, Re-folding, and Purification of Protein Flic (salmonella Enteritidis) with Deletion of Amino Acids 220-320

In order to develop high-performance subunit vaccines, many studies are currently aiming at the development of immunoadjuvants. Flagellin protein (FliC) contained in Salmonella’s filament is one potential candidates for this purpose. However, high antigenic property FliC has made it difficult apply vaccines. A variant with deletion amino acids from 220 320 flagellin been proven have an adjuvant effect lower immunogenicity. present study, we cloned and expressed FliCD220-320 recombinant Escherichia coli system. The expression FliCΔ220-320 was induced by IPTG confirmed SDS-PAGE Western Blot probed 6xHis tag antibody. Results showed that most insoluble. targeted then solubilized, re-folded purified affinity chromatography method purity 80 %. With these results, successfully a source evaluation immune adjuvants.